Tuesday, 7 April 2015

Want to learn about Saliva Testing? Read the Salimetrics comprehensive guides

Advancing Research Through Knowledge

Ready to add salivary measures to your research? Read Salimetrics' key guides in order to gain a basic understanding of using saliva and its relevant biological data for your research.


Recommended Reading 


Description: Start here to learn about the global salivary research network, salivary analytes, and the basic steps to incorporate saliva into your research study. Also identify how Salimetrics can assist you throughout your project life-cycle. 


Description: Then view this brochure for an overview of salivary analytes in research and the research tools we provide to help you in the analyte selection process. 


Description: Soon you'll be ready to start collecting saliva, but make sure you collect right! View this brochure for an overview of recommended collection volumes, Salimetrics tools for researchers, and available collection devices. 


Description: If you want accurate results, make sure you choose a reliable testing lab! In this brochure, we’ve put together a list of features and services to look for when searching for your saliva testing lab 

Length: 13 Pages

Description: Learn the proper techniques and methods for saliva collection, handling, and storage with this pocket guide 

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Sunday, 29 March 2015

Interesting new paper: Methodological Considerations for Hair Cortisol Measurements in Children

Methodological Considerations for Hair Cortisol Measurements in Children 

Abstract
Background:
Hair cortisol levels are used increasingly as a measure for chronic stress in young children. We propose modifications to the current methods used for hair cortisol analysis to more accurately determine reference ranges for hair cortisol across different populations and age groups.

Author information
Slominski R1, Rovnaghi CR, Anand KJ.

1Pain Neurobiology Laboratory, Neuroscience Institute, University of Tennessee Health Science Center, Memphis, Tennessee; 2Division of Pediatric Critical Care Medicine, Department of Pediatrics, University of Tennessee Health Science Center and Le Bonheur Children's Hospital, Memphis, Tennessee

Methods:
The authors compared standard (finely cutting hair) vs. milled methods for hair processing (n=16), developed a 4-step extraction process for hair protein and cortisol (n=16), and compared liquid chromatography-mass spectrometry (LCMS) vs. ELISA assays for measuring hair cortisol (n=28). The extraction process included sequential incubations in methanol and acetone, repeated twice. Hair protein was measured via spectrophotometric ratios at 260/280 nm to indicate the hair dissolution state using a BioTek® plate reader and dedicated software. Hair cortisol was measured using an ELISA assay kit. Individual (n=13), pooled hair samples (n=12) with high, intermediate, and low cortisol values and the ELISA assay internal standards (n=3) were also evaluated by LCMS.

Results:
Milled and standard methods showed highly correlated hair cortisol (rs=0.951, p<0.0001) and protein values (rs=0.902, p=0.0002), although higher yields of cortisol and protein were obtained from the standard method in 13/16 and 14/16 samples respectively (p<0.05). Four sequential extractions yielded additional amounts of protein (36.5%, 27.5%, 30.5%, 3.1%) and cortisol (45.4%, 31.1%, 15.1%, 0.04%) from hair samples. Cortisol values measured by LCMS and ELISA were correlated (rs=0.737; p<0.0001), although cortisol levels (median [IQR]) detected in the same samples by LCMS (38.7 [14.4, 136] ng/ml) were lower than by ELISA (172.2 [67.9, 1051] ng/ml). LCMS also detected cortisone, which comprised 13.4% (3.7%, 25.9%) of the steroids detected.

Conclusion:
Methodological studies suggest that finely cutting hair with sequential incubations in methanol and acetone, repeated twice, extracts greater yields of cortisol than does milled hair. Based on these findings, at least three incubations may be required to extract most of the cortisol in human hair samples. In addition, ELISA-based assays showed greater sensitivity for measuring hair cortisol levels than LCMS-based assays.

Salimetrics offers Hair Cortisol Analysis at our Cambridge Laboratory, should you wish to learn more e mail: europe@salimetrics.com in addition you may wish to use the following links for more information on this growing technology











Monday, 16 March 2015

Salimetrics will again this year sponsor and exhibit at the BPS Psychobiology Section, Annual Conference - Low Wood Windermere, 2nd to 4th September 2015



The BPS Psychobiology Section, Annual Conference, Low Wood Windermere

Low Wood Hotel, overlooking Lake Windermere has fantastic conference and dining facilities, well-equipped en-suite rooms, a health spa and water-sport facilities. It is also only a short distance from the Langdale pikes for beautiful walks and breath-taking views


"where the science is as good as the view"

The meeting is open to all researchers and scientists interested in psychobiology, providing a forum for networking, showcasing research findings and keeping up to date with the latest developments in the field.

Features of the meeting this year include:

Outstanding guest lecturers: 



Professor Michael Maier

Professor Maier is a Consultant Psychiatrist, Director of Medical Education and Head of London School of Psychiatry. He is the Curator of the Corsellis Brain Collection. The collection contains a large number of brains from patients who had psychiatric and neurodegenerative illnesses, but also a series of brains from patients who did not have any brain disease and who therefore provide a very useful control population.






Professor Brian Hughes

Professor Hughes, of the School of Psychology at the National University of Ireland, Galway. His research and publications have focused on psychological stress (particularly its impact on cardiovascular psychophysiology, immunity, and health) and on psychosocial moderators of stress processes (such as social support, cognition, and personality). His work on how the human cardiovascular response habituates across repeated stress exposures has been extensively cited. He also writes widely on the psychology of empiricism and of empirically disputable claims, especially as they pertain to science, health, and medicine.

Non-parallel oral and poster sessions

Two free places for postgraduate students (includes registration and accommodation)
One free undergraduate student place via the undergraduate project prize sponsored by Salimetrics (includes registration and accommodation)

Special 24 hour event for postgraduates at the very competitive price of £62.40
Conference meal, late bar and entertainment on Thursday 3rd September 2015

Submissions
Submissions are invited for the Psychobiology Section Annual Scientific Meeting 2015.
If you are interested in presenting an oral presentation or poster at the conference then please make your submission via the the submissions webpage icon by 23:59 Friday 10th July 2015.

Two free (sponsored) places for Postgraduate Students
The Section is funding two free places at our Annual Scientific Meeting (ASM) for registered postgraduate students. Postgraduates wishing to be awarded one of these places should submit a summary (maximum 1,000 words) concerning the work from which their submission arises in addition to an abstract. Please submit the summary to Dr Richard Stephens at r.stephens@keele.ac.uk email icon .

Entries for these places must be received by 23:59 on 10th July 2015. However, should any places remain unfilled beyond this date then at the discretion of the committee the additional awards may be made on a first come first served basis. These awards cover the registration fee and accommodation at the Low Wood.