The Salimetrics™ Salivary Melatonin Assay Kit is a competitive immunoassay specifically designed and validated for the quantitative determination of melatonin in saliva for research or circadian/biorhythm assessment. The Salimetrics propriety ELISA format allows melatonin assay results in four hours or less with a single sample and one wash step. Salimetrics salivary assay kits are expertly designed, developed and validated to ensure accuracy in saliva and proven to deliver precision results for salivary biomarkers. Our specifically designed antibody promises precise and accurate results in less time.
Melatonin (N-acetyl-5-methoxytryptamine) is a compound secreted mainly by the pineal gland, but synthesized also in many other tissues and cells. In humans, nocturnally peaking oscillations of melatonin are involved in sleep-wakefulness where melatonin concentrations are lower during the day. In recent years, the role of melatonin and its metabolites have been identified as potent, broad acting antioxidants and free radical scavengers in addition to a playing a role in the upregulation of antioxidant enzymes. Melatonin levels in plasma are paralleled by corresponding variations in saliva where the saliva concentrations are about 30% of that found in plasma. Measurement of salivary melatonin is advantageous, especially to avoid invasive venipuncture procedures.
Melatonin ELISA Principle
This is a competitive immunoassay kit. Melatonin in standards and samples compete with Melatonin conjugated to horseradish peroxidase for the antibody binding sites on a microtitre plate. After incubation, unbound components are washed away. Bound Melatonin Enzyme Conjugate is measured by the reaction of the horseradish peroxidase enzyme to the substrate tetramethylbenzidine (TMB). This reaction produces a blue color. A yellow color is formed after stopping the reaction with an acidic solution. The optical density is read on a standard plate reader at 450 nm. The amount of Melatonin Enzyme Conjugate detected is inversely proportional to the amount of Melatonin present in the sample.
Please read the complete kit insert before performing this assay.
Target Analyte: Melatonin
Catalog number: 1-3402 (5PK 1-3402-5)
Testing protocol: Download PDF
Format: 96-well plate
Calibrator range: 0.78-50 pg/mL
Sensitivity: 1.37 pg/mL
Saliva volume/test: 100 µL
Incubation time: 3 hours
Tests per kit: 76 (singlet)
Correlation with serum: 0.81
Controls included in kit? Yes
European Pricing: £389 (€544.60) per Kit
Have a question, contact email@example.com