Tuesday, 14 October 2014

Starting your Degree? Will you be using the ELISA Test? here Salimetrics details some very useful tips (How To)

The Latest Saliva Research Advice and Advisories:

As technology emerges we may discover new applications and limitations of salivary measures; but you can operate on the cutting edge with the most recent advice from the team at Salimetrics. 








Below you will find the Saliva Collection Handbook, which features the most relevant saliva collection and handling information, as well as a number of biomarker-specific articles.

Select your biomarkers of interest to determine which articles are most relevant to your research.



The ABC of ELISA

Saliva Collection Handbook

















Still need to learn more, try one of our Spit Camp Courses
We offer courses within the USA, UK-Europe and Australia

Here is an example of what to expect

Monday, 13 October 2014

Learn Salivary Bioscience from the Experts, today Professor Douglas Granger; Foundation Professor, and Director; Arizona State University, Institute for Interdisciplinary Salivary Bioscience Research (IISBR) Founder and Chief Scientific Strategy Advisor of Salimetrics LLC


Dr. Douglas A. Granger is a psychoneuroendocrinology researcher who is well known for his development of methods related to saliva collection and analysis and the theoretical and statistical integration of salivary measures into developmental research. At Arizona State University, he is a Foundation Professor in the Department of Psychology. 









Douglas also holds an Adjunct Faculty position at the Johns Hopkins University School of Nursing, School of Medicine, and Bloomberg School of Public Health. His studies have been instrumental in the conceptualization and analysis of biosocial relationships involving child well-being, parent-child and family relationships, as well as how these biosocial links moderate and mediate the effects of early adversity and stress on children’s adjustment. Dr. Granger is a leading expert engaged in work focused on the discovery, measurement, and application of analytes (hormones, antibodies, chemicals, DNA) in saliva. 

He has published more than 170 studies and is also a faculty scholar-entrepreneur. Early in his career, Dr. Granger transferred technology, founded and served as President of Salimetrics LLC*, a salivary laboratory and product development company. At Arizona State University, he has created and is the Director of the Institute for Interdisciplinary Salivary Bioscience Research (IISBR), which facilitates the integration of salivary analytes into prevention science, nursing, public health, and medicine.

*In the interest of full disclosure, Dr. Granger is founder and Chief Scientific and Strategy Advisor of Salimetrics LLC (State College, PA), and this relationship is managed by the policies of Arizona State University.

Areas of Scholarly Expertise and Interest

-Research methods, design, and statistics
-Interactions between behavior, stress hormones, and immune function and their relations   to developmental psychopathology
-Non-invasive measurement of endocrine, immune, and disease-related biomarkers and analytes in saliva
-Social forces as mediators and moderators of biobehavioral relationships
-The influence of context on development through biological mechanisms.
For more information, please visit the Institute for Interdisciplinary Salivary Bioscience Research Linked HERE


To read recent publications from Professor Granger please refer to ResearchGate Linked HERE

Tuesday, 7 October 2014

Salimetrics supporting Stoptober, The 28 Day October stop smoking challenge: here we review our Salivary Cotinine Assay









When nicotine from tobacco smoke is taken into the lungs and enters the bloodstream, the principle metabolite produced in the liver is cotinine.  Cotinine diffuses easily from blood into saliva, and salivary and blood levels are highly correlated. (1)  Cotinine in saliva has a longer half-life than nicotine (more than 10 hours), and the literature has documented it to be a specific and sensitive marker for determining exposure to tobacco and nicotine. (2,3,4)​


Salimetrics salivary assay kits are expertly designed, developed and validated to ensure accuracy in saliva and proven to deliver precision results for saliva biomarkers.The Salimetrics Salivary Cotinine Enzyme Kit is a competitive immunoassay designed and validated for the quantitative measurement of cotinine in saliva samples. 

This kit may be used to measure primary or secondhand exposure to nicotine. The detection of exposure to tobacco smoke by measurement of cotinine is the preferred method, as nicotine is not considered a valid marker of smoking status due to its relatively short half-life. Salimetrics has designed this quantitative research tool to provide biomedical researchers with a highly sensitive means to quantify differences in inter-individual cotinine level. 

These differences include factors related to intrinsic and extrinsic predispositions that affect the physiology of nicotine metabolism, the dose of nicotine present in the cigarette (or alternative source), and health behaviours relevant to how cigarettes are smoked (e.g., vent blocking, duration and frequency of puffs).



Saliva Collection Advice - Cotinine
Recent Research, Boston USA


Contact europe@salimetrics.com to talk about the supply of Salivary Assay Kits, Saliva Collection Advice or our Saliva Testing Service. 


1. Benowitz, N.L. (1996). Cotinine as a biomarker of environmental tobacco smoke exposure.  Epidemiol Rev, 18(2), 188-204.

2. Dhar, P. (2004). Measuring tobacco smoke exposure: Quantifying nicotine/cotinine concentration in biological samples by colorimetry, chromatography and immunoassay methods. J Pharm Biomed Anal, 35(1), 155- 68.

3. Alterman, A.I., Gariti, P., Niedbala, R.S. (2002). Varying results for immunoassay screening kits for cotinine levels. Psychol Addict Behav, 16(3), 256-59.


4. Van Vunakis, H., Tashkin, D.P., Rigas, B., et al. (1989). Relative sensitivity and specificity of salivary and serum cotinine in identifying tobacco-smoking status of self-reported non-smokers and smokers of tobacco and/or marijuana. Arch Environ  Health, 44(1), 53-58.