Monday, 6 July 2015

Researching Sleep, Chronobiology? Salimetrics Salivary Melatonin ELISA Kit will give you results in just 4 Hours, best commercialy available

The Salimetrics™ Salivary Melatonin Assay Kit is a competitive immunoassay specifically designed and validated for the quantitative determination of melatonin in saliva for research or circadian/biorhythm assessment. The Salimetrics propriety ELISA format allows melatonin assay results in four hours or less with a single sample and one wash step. Salimetrics salivary assay kits are expertly designed, developed and validated to ensure accuracy in saliva and proven to deliver precision results for salivary biomarkers. Our specifically designed antibody promises precise and accurate results in less time. 

Melatonin (N-acetyl-5-methoxytryptamine) is a compound secreted mainly by the pineal gland, but synthesized also in many other tissues and cells. In humans, nocturnally peaking oscillations of melatonin are involved in sleep-wakefulness where melatonin concentrations are lower during the day. In recent years, the role of melatonin and its metabolites have been identified as potent, broad acting antioxidants and free radical scavengers in addition to a playing a role in the upregulation of antioxidant enzymes. Melatonin levels in plasma are paralleled by corresponding variations in saliva where the saliva concentrations are about 30% of that found in plasma. Measurement of salivary melatonin is advantageous, especially to avoid invasive venipuncture procedures.​

Melatonin ELISA Principle


This is a competitive immunoassay kit. Melatonin in standards and samples compete with Melatonin conjugated to horseradish peroxidase for the antibody binding sites on a microtitre plate. After incubation, unbound components are washed away. Bound Melatonin Enzyme Conjugate is measured by the reaction of the horseradish peroxidase enzyme to the substrate tetramethylbenzidine (TMB). This reaction produces a blue color. A yellow color is formed after stopping the reaction with an acidic solution. The optical density is read on a standard plate reader at 450 nm. The amount of Melatonin Enzyme Conjugate detected is inversely proportional to the amount of Melatonin present in the sample.





Please read the complete kit insert before performing this assay.

Target Analyte:                 Melatonin
Catalog number:              1-3402 (5PK 1-3402-5)
Testing protocol:              Download PDF
Format:                            96-well plate
Type:                                Quantitative
Method:                           ELISA/EIA
Calibrator range:              0.78-50 pg/mL


Sensitivity:                        1.37 pg/mL
Saliva volume/test:           100 µL
Incubation time:                3 hours
Tests per kit:                     76 (singlet)
Correlation with serum:    0.81
Controls included in kit?  Yes

European Pricing: £389 (€544.60) per Kit

Have a question, contact europe@salimetrics.com

Wednesday, 10 June 2015

Salimetrics launch the first Salivary Uric Acid Assay Kit


Noninvasive Biomarker Assessment to Quantitatively Determine Uric Acid Levels

Researching Fanconi Syndrome, Lesch-Nythan Syndrome, Coronary Artery Disease, Hyperuricemia, Dementia, Optic Neuritis, Hypouricemia, Preeclampsia, Prehypertension, BMI and Obesity, Type 2 Diabetes, Metabolic Syndrome, Uric Acid, Kidney Stones, Multiple Sclerosis, Cardiovascular Disease, Parkinsons Disease, Kidney Disease, Antioxidant Activity, Gout, Alzheimers Disease, Oxidative Stress,,,,,


Shipping from June 15th,  contact details below

Link to the Protocol

The assay is different from the majority of the Salimetrics assays, most of which are competitive ELISAs. The Uric Acid assay protocol is different as it is an enzymatic assay, but it still uses standards of known concentration to determine the concentration in the sample, much like our ELISA kits, without the need for a standard curve. The current price in tghe UK is £209 for the 96 well assay kit


View Press Release     View Specifications

USA

Europe 


Australia / NZ

Monday, 8 June 2015

Salimetrics Spit Camp - Comprehensive training for Saliva Collection, Saliva Testing and Laboratory Practise


Having created a comprehensive range of Salivary Assay Kits and Saliva Collection Devices, recognised as the best commercially available, Salimetrics also offer unrivalled training days within the USA, Europe and Australia






Affectionately known as "Spit Camps" the training days include expert tuition on Saliva Sample Collection and Handling, Health and Safety and Saliva Storage, as well as the theory behind Immuno- and Kinetic Salivary Assays and practical instruction and advice on how to get the best from your assays.

We are often asked about the content and cost of our Cambridge Training Days, here we publish the information pack sent to all participants. It includes instructions for collecting your own saliva for practical testing during the day, information on places to stay, a pre-course questionnaire and required consents.

We plan to run training days each month this year. Dates are published HERE 

This year we are offering an extra half day tuition which is lecture based covering additional theory. To book your place within Europe, email: europe@salimetrics.com

Within the USA we offer Spit Camp at Arizona State University and various other locations, to view the full list follow this LINK










Research and Diagnostic Salivary Assay Kits & Protocols from Salimetrics

Why do researchers consider Salimetrics assays the standard of comparison? 


We offer the most reliable, repeatable and most accurate results in saliva, because we are focused on science driven by rigorous, uncompromising testing.






Saliva is our specialty. No modified blood or urine assays here—Salimetrics assays are designed specifically for salivary testing by pioneers in salivary bioscience, and are now recognized globally as the standard in saliva testing. Choose Salimetrics. Your research is too important to settle for anything less.

As part of our continuous improvement programme out of Carlsbad, California we have just launched a modified Salivary Melatonin Assay and  in July our new Salivary Uric Acid Assay will start in production and commence shipments

We publish the full testing protocol and Kit Specifications on our website. Link Here
 http://www.salimetrics.com/assay-kits


We of course have on offer a full range of validated Saliva Collection Methods, all published on our website. Link Here:


Got a question? Ask an expert: europe@salimetrics.com

Tel: +44 (0) 1638 782619

Pipetting Proficiency, test your skills with the Salimetrics Method and Teaching Aid



Pipetting variance can lead to errors which can result in:

– Unrecognized wrong results
– Failure to pass a good product (as in QC)
– Failure to fail a poor product (as in QC)

Pipetting variance can be attributed to:

– Poorly seated tips
– Variable viscosities not taken into account
– Profusion(residual volume on exterior of tip) carry-over
– Pipette out of calibration (dropped or overly used)

– Inexperience or need for retraining

Take the test with your staff and use this model for Training


Need more training, Come to a Spit Camp